Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11912(B)] to IGFBP7
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-IGFBP7 antibody [EPR11912(B)]
See all IGFBP7 primary antibodies
DescriptionRabbit monoclonal [EPR11912(B)] to IGFBP7
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IGFBP7 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16270
- SW480, 293T and MCF7 cell lysates; Human pancreas, prostate and lung cancer tissues; MCF7 cells; 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab171085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
- Information by UniProt
- AGM antibody
- Angiomodulin antibody
- FSTL2 antibody
All lanes : Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
This image was produced using unpurified antibody.
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with Purified ab171085 at 1:100 (6.0 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with purified ab171085 at 1:60 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/10000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IGFBP7 with purified ab171085 at 1:250 dilution (2.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Flow cytometric analysis of permeabilized 293T cells labeling IGFBP7 with ab171085 at 1/250 dilution (red) compared to a rabbit IgG negative control (green). This image was produced using unpurified antibody.
Immunofluorescent analysis of MCF7 cells labeling IGFBP7 with ab171085 at 1/250 dilution. This image was produced using unpurified antibody.
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.
ab171085 has been referenced in 2 publications.
- Ong CJ et al. An Optimised Protocol Harnessing Laser Capture Microdissection for Transcriptomic Analysis on Matched Primary and Metastatic Colorectal Tumours. Sci Rep 10:682 (2020). PubMed: 31959771
- Sill M et al. Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays. Microarrays (Basel) 5:N/A (2016). IHC . PubMed: 27600085