Recombinant
RabMAb

Recombinant Anti-IGFBP7 antibody [EPR11912(B)] (Alexa Fluor® 647) (ab214262)

Overview

  • Product name

    Anti-IGFBP7 antibody [EPR11912(B)] (Alexa Fluor® 647)
    See all IGFBP7 primary antibodies
  • Description

    Rabbit monoclonal [EPR11912(B)] to IGFBP7 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human IGFBP7 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16270

  • Positive control

    • ICC/IF: MCF-7 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in MCF-7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGM antibody
    • Angiomodulin antibody
    • FSTL2 antibody
    • IBP-7 antibody
    • IBP7 antibody
    • IBP7_HUMAN antibody
    • IGF binding protein 7 antibody
    • IGF-binding protein 7 antibody
    • IGFBP rP1 antibody
    • IGFBP-7 antibody
    • IGFBP-rP1 antibody
    • IGFBP7 antibody
    • IGFBPRP1 antibody
    • Insulin like growth factor binding protein 7 antibody
    • Insulin-like growth factor-binding protein 7 antibody
    • MAC25 antibody
    • MAC25 protein antibody
    • PGI2 stimulating factor antibody
    • PGI2-stimulating factor antibody
    • Prostacyclin stimulating factor antibody
    • Prostacyclin-stimulating factor antibody
    • PSF antibody
    • RAMSVPS antibody
    • TAF antibody
    • Tumor-derived adhesion factor antibody
    see all

Images

  • Ab214262 staining IGFBP7 in MCF-7 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab214262 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

    This product also gave a positive signal under the same testing conditions in MCF-7 cells fixed with 4% formaldehyde (10min).

References

ab214262 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214262.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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