Anti-Human IgG FC fragment antibody [EM-07] (ab77118)
Key features and details
- Mouse monoclonal [EM-07] to Human IgG FC fragment
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Human IgG FC fragment antibody [EM-07]
See all Human IgG FC fragment primary antibodies -
Description
Mouse monoclonal [EM-07] to Human IgG FC fragment -
Host species
Mouse -
Specificity
Reacts with Fc part of human IgG heavy chain and with isolated Fc fragments. It does not cross-react with IgM. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human Human IgG FC fragment.
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Positive control
- Flow Cyt: Human peripheral whole blood,
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
EM-07 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
Target
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Relevance
The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.
Images
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Flow cytometry multicolor surface staining of human peripheral whole blood stained with ab77118 (concentration in sample 1 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).
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Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained with ab77118 (concentration in sample 1 μg/ml, GAM APC). The pattern varies depending on the blood donor.
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Flow cytometry surface staining pattern of human peripheral whole blood with ab77118 (concentration in sample 1 μg/ml, GAM APC). The pattern varies depending on the blood donor.
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Flow cytometry surface staining pattern of human peripheral whole blood with ab77118 purified antibody (concentration in sample 1 μg/ml, GAM APC). The pattern varies depending on the blood donor.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab77118 has been referenced in 2 publications.
- Swartzlander DB et al. Identification of SUMO modification sites in the base excision repair protein, Ntg1. DNA Repair (Amst) 48:51-62 (2016). PubMed: 27839712
- Scotti L et al. Inhibition of angiopoietin-1 (ANGPT1) affects vascular integrity in ovarian hyperstimulation syndrome (OHSS). Reprod Fertil Dev 28:690-9 (2016). PubMed: 25485810