Recombinant

Recombinant Anti-IgG antibody [IG507R] - BSA and Azide free (ab218891)

Overview

  • Product name

    Anti-IgG antibody [IG507R] - BSA and Azide free
    See all IgG primary antibodies
  • Description

    Rabbit monoclonal [IG507R] to IgG - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab218891 does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IgG. Purified human Ig Gamma Chain. UniProt: P01857, P01859, P01860, P01861

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • This data was produced with ab218427, the same antibody in a different formulation with BSA and Azide.
    ab218427 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemical analysis of formalin fixed, paraffin embedded human tonsil tissue labeling IgG with ab218891 at 1 µg/mL.

References

ab218891 has not yet been referenced specifically in any publications.

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