Recombinant

Recombinant Anti-IgG antibody [R10Z8E9] (ab124055)

Submit a review Q&A (3)References (1)
Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)
  • ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)
  • Anti-IgG antibody [R10Z8E9] (ab124055)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [R10Z8E9] to IgG
  • Suitable for: WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-IgG antibody [R10Z8E9]
    See all IgG primary antibodies

  • Description

    Mouse monoclonal [R10Z8E9] to IgG

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • ELISA: Human IgG1. WB: Human serum lysate.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 21st September 2020.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    R10Z8E9

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 36 kDa.
ELISA
Use at an assay dependent concentration.
Notes
WB
1/1000. Predicted molecular weight: 36 kDa.
ELISA
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    Images

    • Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)
      Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)
      Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution + Human serum lysate at 10 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366)

      Predicted band size: 36 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Expected MW: 50 kDa.

    • ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)
      ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)

      50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes. 

    • Anti-IgG antibody [R10Z8E9] (ab124055)
      Anti-IgG antibody [R10Z8E9] (ab124055)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab124055? Please let us know so that we can cite the reference in this datasheet.

    ab124055 has been referenced in 1 publication.

    • Hou L  et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    I'm looking for an anti-human IgG antibody that will not cross-react with cynomolgus monkey.

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    Answer

    Thank you for your inquiry.

    I found a mouse monoclonal to Human IgG that does not react with cynomolgus monkey as ab124055.

    https://www.abcam.com/igg-antibody-r10z8e9-ab124055.html

    I hope this information helps. Please contact us with any other questions.

    Read More

    Question



    Could you please send me the certificate of analysis of product “Anti-IgG antibody [R10Z8E9] (ab124055)” from Abcam.

    Product name: Anti-IgG Antibody (mouse anti-human) (Clone R10Z8E9)

    Catalogue # ab124055

    Vendor lot# ***********

    Read More
    Answer

    Thank you for contacting Abcam.

    Please find attached the Certificate of Compliance for ab124055.

    If there is anything else I can help you with, please let me know.

    Read More

    Question

    Inquiry: Would it be possible to get a Certificate of Analysis or a Certificate of compliance for the following product: Anti-IgG antibody [R10Z8E9] (ab124055) with lot GR73298-4.

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    Answer

    Thank you for contacting Abcam regarding ab124055.


    Attached please find the Certificate of Compliance you requested.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com