Recombinant Anti-IgG antibody [R10Z8E9] (ab124055)
![Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)](https://www.abcam.com/ps/products/124/ab124055/Images/ab124055-412863-AntiIgG-antibody-R10Z8E9-western-blot-serum-human.jpg "Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [R10Z8E9] to IgG
- Suitable for: WB, ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IgG antibody [R10Z8E9]
See all IgG primary antibodies -
Description
Mouse monoclonal [R10Z8E9] to IgG -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ELISA: Human IgG1. WB: Human serum lysate.
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General notes
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
R10Z8E9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 36 kDa.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 36 kDa. |
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ELISA
Use at an assay dependent concentration. |
Target
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Cellular localization
Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- SwissProt: P01857 Human
- SwissProt: P01859 Human
- SwissProt: P01860 Human
- SwissProt: P01861 Human
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Alternative names
- Ig gamma 1 chain C region antibody
- Ig gamma 2 chain C region antibody
- Ig gamma 3 chain C region antibody
see all
Images
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Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution + Human serum lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366)
Predicted band size: 36 kDa
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Expected MW: 50 kDa.
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![ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)](https://www.abcam.com/ps/products/124/ab124055/Images/ab124055-412862-AntiIgG-antibody-R10Z8E9-elisa-human1.jpg)
ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
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![Anti-IgG antibody [R10Z8E9] (ab124055)](https://www.abcam.com/ps/products/124/ab124055/Images/ab124055-5-benefits-of-recombinant-antibodies.png)
Anti-IgG antibody [R10Z8E9] (ab124055)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124055 has been referenced in 3 publications.
- Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
- Zhang J et al. RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and Chrm2 receptor gene expression in primary sensory neurons. J Biol Chem 293:19078-19091 (2018). PubMed: 30327427
- Laumet G et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat Neurosci 18:1746-55 (2015). PubMed: 26551542