Key features and details
- Mouse monoclonal [rIG266] to IgG
- Suitable for: Protein Array, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IgG antibody [rIG266]
See all IgG primary antibodies
DescriptionMouse monoclonal [rIG266] to IgG
Tested applicationsSuitable for: Protein Array, IHC-Pmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human IgG. Purified human Ig Gamma Chain.
- IHC-P: Human tonsil tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate by Protein A/G.
Our Abpromise guarantee covers the use of ab238004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT.
- Ig gamma 1 chain C region antibody
- Ig gamma 2 chain C region antibody
- Ig gamma 3 chain C region antibody
ab238004 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG using ab238004 at 1 μg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238004 has been referenced in 1 publication.
- Shi S et al. Ras-ERK signalling represses H1.4 phosphorylation at serine 36 to promote non-small-cell lung carcinoma cells growth and migration. Artif Cells Nanomed Biotechnol 47:2343-2351 (2019). PubMed: 31184227