Overview

  • Product name

    Anti-IgG antibody [RIGG-69]
    See all IgG primary antibodies
  • Description

    Rabbit monoclonal [RIGG-69] to IgG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein corresponding to Rat IgG.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    RIGG-69
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.

For unpurified, use 1/200 - 1/1000.

ELISA 1/1000 - 1/10000.

Target

Images

  • All lanes : purified at 1/1000 dilution

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : Human heart tissue lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Direct ELISA antibody dose-response curve using ab133470 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • All lanes : unpurified at 1/150 dilution

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : Human heart tissue lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Antibody dose-response curve using purified ab133470. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • All lanes : Anti-IgG antibody [RIGG-69] (ab133470) (unpurified)

    Lane 1 : Rat spleen
    Lane 2 : Human tonsil
  • Antibody dose-response curve using unpurified ab133470. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

References

This product has been referenced in:

  • Lin H  et al. LncRNA SUMO1P3 promotes proliferation and inhibits apoptosis in colorectal cancer by epigenetically silencing CPEB3. Biochem Biophys Res Commun 511:239-245 (2019). Read more (PubMed: 30799082) »
  • Bartolák-Suki E  et al. Optimization of Variable Ventilation for Physiology, Immune Response and Surfactant Enhancement in Preterm Lambs. Front Physiol 8:425 (2017). Read more (PubMed: 28690548) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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