Recombinant Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-IgG1 + IgG2a + IgG3 antibody [M204-3]
See all IgG1 + IgG2a + IgG3 primary antibodies -
Description
Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within IgG1 + IgG2a + IgG3. The exact sequence is proprietary.
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Positive control
- WB: Brain (Mouse) Cytoplasmic Lysate - normal tissue (ab30148), Rat plasma, mouse plasma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M204-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000.
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ELISA |
Use a concentration of 0.015 - 1 µg/ml.
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Notes |
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WB
1/2000 - 1/10000. |
ELISA
Use a concentration of 0.015 - 1 µg/ml. |
Target
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Cellular localization
Secreted
Images
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Direct ELISA antibody dose-response curve using ab133469 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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Antibody dose-response curve using ab133469. Antigen concentration of 1µg/ml. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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All lanes : Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
Lane 1 : Rat plasma
Lane 2 : Mouse plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab133469 has been referenced in 23 publications.
- Yen HL et al. Cellular tropism of SARS-CoV-2 in the respiratory tract of Syrian hamsters and B6.Cg-Tg(K18-ACE2)2Prlmn/J transgenic mice. Vet Pathol 59:639-647 (2022). PubMed: 34467820
- Antman-Passig M et al. Optical Nanosensor for Intracellular and Intracranial Detection of Amyloid-Beta. ACS Nano 16:7269-7283 (2022). PubMed: 35420796
- Martin H et al. Usutu Virus Infects Human Placental Explants and Induces Congenital Defects in Mice. Viruses 14:N/A (2022). PubMed: 35893684
- Wilson MN et al. Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex. Nat Commun 13:7945 (2022). PubMed: 36572698
- Dijkstra KK et al. Patient-Derived Organoid Models of Human Neuroendocrine Carcinoma. Front Endocrinol (Lausanne) 12:627819 (2021). PubMed: 33776923