Product nameAnti-IgG1 + IgG2a + IgG3 antibody [M204-3]
See all IgG1 + IgG2a + IgG3 primary antibodies
DescriptionRabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide within IgG1 + IgG2a + IgG3. The exact sequence is proprietary.
- WB: Brain (Mouse) Cytoplasmic Lysate - normal tissue (ab30148), Rat plasma, mouse plasma.
Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|ELISA||Use a concentration of 0.015 - 1 µg/ml.|
Direct ELISA antibody dose-response curve using ab133469 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Antibody dose-response curve using ab133469. Antigen concentration of 1µg/ml. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
All lanes : Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
Lane 1 : Rat plasma
Lane 2 : Mouse plasma
Lysates/proteins at 10 µg per lane.
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking buffer and concentration: 5% NFDM/TBST.
This product has been referenced in:
- Lorenzo-Martín LF et al. Vagal afferents contribute to sympathoexcitation-driven metabolic dysfunctions. J Endocrinol 240:483-496 (2019). Read more (PubMed: 30703063) »
- Novati A et al. Sexual behavior and testis morphology in the BACHD rat model. PLoS One 13:e0198338 (2018). Read more (PubMed: 29883458) »