Recombinant
RabMAb

Recombinant Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

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Overview

  • Product name

    Anti-IgG1 + IgG2a + IgG3 antibody [M204-3]
    See all IgG1 + IgG2a + IgG3 primary antibodies

  • Description

    Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide within IgG1 + IgG2a + IgG3. The exact sequence is proprietary.

  • Positive control

    • WB: Brain (Mouse) Cytoplasmic Lysate - normal tissue (ab30148), Rat plasma, mouse plasma.

  • General notes

    Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    M204-3

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
ELISA Use a concentration of 0.015 - 1 µg/ml.

Target

  • Cellular localization

    Secreted

Images

  • ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

    Direct ELISA antibody dose-response curve using ab133469 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

    Antibody dose-response curve using ab133469. Antigen concentration of 1µg/ml. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    All lanes : Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

    Lane 1 : Rat plasma
    Lane 2 : Mouse plasma

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

See all 6 Publications for this product

Publishing research using ab133469? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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