For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    igg1--igg2a--igg3-antibody-m204-3-ab133469.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email
RecombinantRabMAb

Recombinant Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

  • Datasheet
Submit a review Submit a question References (19)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
  • ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
  • Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
  • Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Alexa Fluor® 488 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab185033)
Primary
Product image
Anti-mouse IgG Fc antibody [RMG06] (ab197780)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-IgG1 + IgG2a + IgG3 antibody [M204-3]
    See all IgG1 + IgG2a + IgG3 primary antibodies
  • Description

    Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within IgG1 + IgG2a + IgG3. The exact sequence is proprietary.

  • Positive control

    • WB: Brain (Mouse) Cytoplasmic Lysate - normal tissue (ab30148), Rat plasma, mouse plasma.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    M204-3
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Associated products

  • Alternative Versions

    • Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] - BSA and Azide free (ab226129)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000.
ELISA
Use a concentration of 0.015 - 1 µg/ml.
Notes
WB
1/2000 - 1/10000.
ELISA
Use a concentration of 0.015 - 1 µg/ml.

Target

  • Cellular localization

    Secreted

Images

  • ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

    Direct ELISA antibody dose-response curve using ab133469 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

    Antibody dose-response curve using ab133469. Antigen concentration of 1µg/ml. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    All lanes : Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

    Lane 1 : Rat plasma
    Lane 2 : Mouse plasma

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

  • Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
    Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (19)

Publishing research using ab133469? Please let us know so that we can cite the reference in this datasheet.

ab133469 has been referenced in 19 publications.

  • Dijkstra KK  et al. Patient-Derived Organoid Models of Human Neuroendocrine Carcinoma. Front Endocrinol (Lausanne) 12:627819 (2021). PubMed: 33776923
  • Will Castro LSEP  et al. The Widely Used Antihelmintic Drug Albendazole is a Potent Inducer of Loss of Heterozygosity. Front Pharmacol 12:596535 (2021). PubMed: 33679394
  • Hendaoui I  et al. Tenascin-W Is a Novel Stromal Marker in Biliary Tract Cancers. Front Immunol 11:630139 (2020). PubMed: 33692777
  • Biery MC  et al. A Protocol for the Generation of Treatment-naïve Biopsy-derived Diffuse Intrinsic Pontine Glioma and Diffuse Midline Glioma Models. J Exp Neurol 1:158-167 (2020). PubMed: 33768215
  • Butt M  et al. Autophagy of Sensory Neurons in the Trigeminal and Dorsal Root Ganglia of Cynomolgus Monkeys (Macaca fascicularis). Toxicol Pathol 48:238-243 (2020). PubMed: 31269872
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab133469.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.