Recombinant Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
![ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)](https://www.abcam.com/ps/products/133/ab133469/Images/ab133469-288992-anti-igg1-igg2a-igg3-antibody-m204-3-elisa.jpg "ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-IgG1 + IgG2a + IgG3 antibody [M204-3]
See all IgG1 + IgG2a + IgG3 primary antibodies -
Description
Rabbit monoclonal [M204-3] to IgG1 + IgG2a + IgG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within IgG1 + IgG2a + IgG3. The exact sequence is proprietary.
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Positive control
- WB: Brain (Mouse) Cytoplasmic Lysate - normal tissue (ab30148), Rat plasma, mouse plasma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M204-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000.
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| ELISA |
Use a concentration of 0.015 - 1 µg/ml.
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| Notes |
|---|
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WB
1/2000 - 1/10000. |
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ELISA
Use a concentration of 0.015 - 1 µg/ml. |
Target
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Cellular localization
Secreted
Images
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ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
Direct ELISA antibody dose-response curve using ab133469 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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![ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)](https://www.abcam.com/ps/products/133/ab133469/Images/ab133469-235599-anti-igg1-igg2a-igg3-antibody-m204-3-elisa.jpg)
ELISA - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)Antibody dose-response curve using ab133469. Antigen concentration of 1µg/ml. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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![Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)](https://www.abcam.com/ps/products/133/ab133469/Images/ab133469-235516-anti-igg1-igg2a-igg3-antibody-m204-3-western-blot.jpg)
Western blot - Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)All lanes : Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
Lane 1 : Rat plasma
Lane 2 : Mouse plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
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![Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)](https://www.abcam.com/ps/products/133/ab133469/Images/ab133469-5-benefits-of-recombinant-antibodies.png)
Anti-IgG1 + IgG2a + IgG3 antibody [M204-3] (ab133469)
Datasheets and documents
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Datasheet download
References (19)
ab133469 has been referenced in 19 publications.
- Dijkstra KK et al. Patient-Derived Organoid Models of Human Neuroendocrine Carcinoma. Front Endocrinol (Lausanne) 12:627819 (2021). PubMed: 33776923
- Will Castro LSEP et al. The Widely Used Antihelmintic Drug Albendazole is a Potent Inducer of Loss of Heterozygosity. Front Pharmacol 12:596535 (2021). PubMed: 33679394
- Hendaoui I et al. Tenascin-W Is a Novel Stromal Marker in Biliary Tract Cancers. Front Immunol 11:630139 (2020). PubMed: 33692777
- Biery MC et al. A Protocol for the Generation of Treatment-naïve Biopsy-derived Diffuse Intrinsic Pontine Glioma and Diffuse Midline Glioma Models. J Exp Neurol 1:158-167 (2020). PubMed: 33768215
- Butt M et al. Autophagy of Sensory Neurons in the Trigeminal and Dorsal Root Ganglia of Cynomolgus Monkeys (Macaca fascicularis). Toxicol Pathol 48:238-243 (2020). PubMed: 31269872
