Product nameAnti-IgG1 antibody
See all IgG1 primary antibodies
DescriptionRabbit polyclonal to IgG1
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IgG1 aa 81-110 (intracellular) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P01857
- Native Human IgG1 protein (ab90283) can be used as a positive control in WB. HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab170461 is purified through a protein A column, followed by peptide affinity purification
Our Abpromise guarantee covers the use of ab170461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/100 - 1/500. Predicted molecular weight: 36 kDa.|
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Cellular localizationSecreted. Cell membrane; Single-pass membrane protein
- Ig gamma 1 chain C region antibody
- IgG heavy chain locus antibody
- IGHG1 antibody
All lanes : Anti-IgG1 antibody (ab170461) at 1/2000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : Human spleen lysate
Lane 4 : Human plasma
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-rabbit IgG
Predicted band size: 36 kDa
Flow cytometry analysis of HepG2 cells labeling IgG1 (green, right histogram), using ab170461 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.
ab170461 has not yet been referenced specifically in any publications.