Product nameAnti-IgG1 antibody [EPR4417]
See all IgG1 primary antibodies
DescriptionRabbit monoclonal [EPR4417] to IgG1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IgG1 aa 50-150 (Hinge). The exact sequence is proprietary.
- Human tonsil and human spleen lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.87 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab108969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Cellular localizationSecreted. Cell membrane; Single-pass membrane protein
- Ig gamma 1 chain C region antibody
- IgG heavy chain locus antibody
- IGHG1 antibody
All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution (unpurified)
Lane 1 : IgG1 at 0.01 µg
Lane 2 : IgG2 at 0.01 µg
Lane 3 : IgG3 at 0.01 µg
Lane 4 : IgG4 at 0.01 µg
Lane 5 : Human tonsil lysate at 10 µg
Lane 6 : Human spleen lysate at 10 µg
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD