Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4417] to IgG1
- Suitable for: WB
- Reacts with: Human
Product nameAnti-IgG1 antibody [EPR4417]
See all IgG1 primary antibodies
DescriptionRabbit monoclonal [EPR4417] to IgG1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IgG1 aa 50-150 (Hinge). The exact sequence is proprietary.
- Human tonsil and human spleen lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.87 x 10 -10 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab108969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Cellular localizationSecreted. Cell membrane; Single-pass membrane protein
- Ig gamma 1 chain C region antibody
- IgG heavy chain locus antibody
- IGHG1 antibody
All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution (unpurified)
Lane 1 : IgG1 at 0.01 µg
Lane 2 : IgG2 at 0.01 µg
Lane 3 : IgG3 at 0.01 µg
Lane 4 : IgG4 at 0.01 µg
Lane 5 : Human tonsil lysate at 10 µg
Lane 6 : Human spleen lysate at 10 µg
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab108969 has been referenced in 1 publication.
- Jing Z et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). PubMed: 29512722