Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4417] to IgG1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-IgG1 antibody [EPR4417] - BSA and Azide free
See all IgG1 primary antibodies
DescriptionRabbit monoclonal [EPR4417] to IgG1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IgG1 aa 50-150 (Hinge). The exact sequence is proprietary.
- WB: Human tonsil and spleen lysates.
Ab232544 is the carrier-free version of ab108969. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232544 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.|
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Cellular localizationSecreted. Cell membrane; Single-pass membrane protein
- Ig gamma 1 chain C region antibody
- IgG heavy chain locus antibody
- IGHG1 antibody
All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108969).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232544 has not yet been referenced specifically in any publications.