Recombinant
RabMAb

Recombinant Anti-IgG1 antibody [EPR4417] - BSA and Azide free (ab232544)

Overview

  • Product name

    Anti-IgG1 antibody [EPR4417] - BSA and Azide free
    See all IgG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4417] to IgG1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IgG1 aa 50-150 (Hinge). The exact sequence is proprietary.

  • Positive control

    • WB: Human tonsil and spleen lysates.
  • General notes

    Ab232544 is the carrier-free version of ab108969. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232544 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4417
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.

Target

  • Relevance

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • Cellular localization

    Secreted. Cell membrane; Single-pass membrane protein
  • Database links

  • Alternative names

    • Ig gamma 1 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG1 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    see all

Images

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)

    Lane 1 : Human tonsil lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108969).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108969).

References

ab232544 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232544.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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