Product nameAnti-IgG1 antibody [EPR4417] - BSA and Azide free
See all IgG1 primary antibodies
DescriptionRabbit monoclonal [EPR4417] to IgG1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IgG1 aa 50-150 (Hinge). The exact sequence is proprietary.
- WB: Human tonsil and spleen lysates.
Ab232544 is the carrier-free version of ab108969. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232544 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.|
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Cellular localizationSecreted. Cell membrane; Single-pass membrane protein
- Ig gamma 1 chain C region antibody
- IgG heavy chain locus antibody
- IGHG1 antibody
All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108969).
ab232544 has not yet been referenced specifically in any publications.