RecombinantRabMAb

Recombinant Anti-IgG2 antibody [EPR4418] (ab134050)

Submit a review Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Flow Cytometry - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
  • Anti-IgG2 antibody [EPR4418] (ab134050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4418] to IgG2
  • Suitable for: WB, IHC-P, Flow Cyt, ELISA, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IgG2 antibody [EPR4418]
    See all IgG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4418] to IgG2

  • Host species

    Rabbit

  • Specificity

    ab134050 does not cross-react with human IgG1, IgG3 or IgG4.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ELISA, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human IgG2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: IgG2 recombinant protein; Human spleen, and Human tonsil lysates. IHC-P: human tonsil and thyroid gland carcinoma tissue. ICC/IF: Raji cells. Flow Cyt: Raji cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4418

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 36 kDa).

For unpurified use at 1/10000-1/20000
IHC-P 1/40000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500-1/1000
Flow Cyt 1/70.
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria.

    • Cellular localization

      Secreted

    • Database links

      • Alternative names

        • Ig gamma-2 chain C region antibody
        • IGHG2 antibody
        • immunoglobulin Gm2 antibody
        • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
        • immunoglobulin heavy constant, gamma 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG2 with purified ab134050 at 1:40000 dilution (0.025 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      • Flow Cytometry - Anti-IgG2 antibody [EPR4418] (ab134050)
        Flow Cytometry - Anti-IgG2 antibody [EPR4418] (ab134050)

        Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling IgG2 with purified ab134050 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (ab134050)
        Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] (ab134050)

        Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma cell line) cells labeling IgG2 with purified ab134050 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)
        Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)
        All lanes : Anti-IgG2 antibody [EPR4418] (ab134050) at 1/5000 dilution (Purified)

        Lane 1 : Human spleen lysates
        Lane 2 : Human tonsil lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 36 kDa
        Observed band size: 52 kDa
        why is the actual band size different from the predicted?

      • Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)
        Western blot - Anti-IgG2 antibody [EPR4418] (ab134050)
        All lanes : Anti-IgG2 antibody [EPR4418] (ab134050) at 1/10000 dilution (Unpurified)

        Lane 1 : Human Recombinant IgG2 at 0.01 µg
        Lane 2 : Human Recombinant IgG1 at 0.01 µg
        Lane 3 : Human Recombinant IgG3 at 0.01 µg
        Lane 4 : Human Recombinant IgG4 at 0.01 µg
        Lane 5 : Human spleen lysate at 10 µg
        Lane 6 : Human tonsil lysate at 10 µg

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 36 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)

        Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IgG2 with unpurified ab134050 at 1/500 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] (ab134050)

        Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue labeling IgG2 with unpurified ab134050 at 1/500 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-IgG2 antibody [EPR4418] (ab134050)
        Anti-IgG2 antibody [EPR4418] (ab134050)

      References (1)

      Publishing research using ab134050? Please let us know so that we can cite the reference in this datasheet.

      ab134050 has been referenced in 1 publication.

      • Jing Z  et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). PubMed: 29512722

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Can you please let me know if any of the following Anti- human IgG2 primary antibodies cross react with Rhesus IgG2?    I am also interested in purchasing Anti- human IgG3 and IgG4  primary antibodies that recognize Rhesus IgG3 and IgG4, respectively.

      Read More
      Answer

      I have reviewed these products and have been in touch with the labs regarding reactivity with Rhesus as requested. We would not recommend any human IgG2 antibody which we know is directed to the hinge region, such as ab1935, against Rhesus as the homology of these is poor. However with this exception, in general there seems to be good homology between Rhesus and Human IgG subtypes. More information about human and Rhesus IgGs may be found at the links below: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1782391/ http://nhpreagents.bidmc.harvard.edu/nhp/macaqueisotypes.aspx http://www.kcl.ac.uk/immunobiology/Mac_ig/ We have not tested any of these products against Rhesus. For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial We could also offer enrollment in the AbTrial program for anti-IgG4 ab109493. We do not currently have any anti-IgG3 primary antibodies. More information about ab109493 can be found at the locations below: Rabbit monoclonal [EP4420] to IgG4: https://www.abcam.com/igg4-antibody-ep4420-ab109493.html

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com