Recombinant
RabMAb

Recombinant Anti-IgG2 antibody [EPR4418] (HRP) (ab209010)

Overview

  • Product name

    Anti-IgG2 antibody [EPR4418] (HRP)
    See all IgG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4418] to IgG2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    ab209010 does not cross-react with Human IgG1, IgG3 or IgG4.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IgG2 aa 50-150. The exact sequence is proprietary.
    Database link: P01859

  • Positive control

    • WB: Human Spleen and Human Tonsil tissue lysates. IHC-P: normal human tonsil tissue sections
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4418
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab209010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Ig gamma-2 chain C region antibody
    • IGHG2 antibody
    • immunoglobulin Gm2 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • immunoglobulin heavy constant, gamma 2 antibody
    see all

Images

  • IHC image of IgG2 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209010, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-IgG2 antibody [EPR4418] (HRP) (ab209010) at 1/5000 dilution

    Lane 1 : Spleen (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Tonsil (Human) Whole Cell Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209010 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209010 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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