Overview

  • Product name
    Anti-IgG2b antibody [KT93] (HRP)
  • Description
    Rat monoclonal [KT93] to IgG2b (HRP)
  • Host species
    Rat
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse IgG2b.

  • Positive control
    • Mouse IgG2b

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab170322 is purified through a Protein G column and labeled with horseradish peroxidase.
  • Clonality
    Monoclonal
  • Clone number
    KT93
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 44 kDa.

Dilution when using DAB as substrate.

ELISA 1/500.

Target

  • Relevance
    IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists oftwo identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • gamma2b antibody
    • Ig gamma-2B chain C region antibody
    • Igh-3 antibody
    • IGHG2B antibody
    • Immunoglobulin G2b antibody
    see all

Images

  • ELISA for crossreactivity to various immunoglobulins.

References

ab170322 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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