Recombinant

Recombinant Anti-IgG3 antibody [RM119] (Biotin) (ab224181)

Overview

  • Product name

    Anti-IgG3 antibody [RM119] (Biotin)
    See all IgG3 primary antibodies
  • Description

    Rabbit monoclonal [RM119] to IgG3 (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Sandwich ELISA, IHC, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Goat
  • Immunogen

    Full length protein corresponding to Human IgG3.
    Database link: P01860

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab224181 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM119
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Use: 50 ng/well – 200 ng/well (for Capture); 0.05 μg/mL – 0.2 μg/mL (for Detection).

IHC Use a concentration of 2 - 5 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    IgG3, like IgG1, activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • DKFZp686H11213 antibody
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Ig gamma 3 chain C region antibody
    • IGHG3 antibody
    • Immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • MGC45809 antibody
    see all

Images

  • A titer ELISA using ab224181. The plate was coated with different amounts of human IgG3. A serial dilution of ab224181 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA of human immunoglobulins shows ab224181 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab224181 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

  • Sandwich ELISA using ab224181 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

  • Sandwich ELISA using ab224181 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.

  • Human lymphoid tissue stained for human IgG3 using ab224181 at 5 µg/ml in immunohistochemical analysis. 

References

ab224181 has not yet been referenced specifically in any publications.

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