Overview

  • Product name
    Anti-IgG4 antibody [EP4420]
    See all IgG4 primary antibodies
  • Description
    Rabbit monoclonal [EP4420] to IgG4
  • Host species
    Rabbit
  • Specificity
    Does not cross-react with IgG1, IgG2, or IgG3.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IgG4 aa 50-150 (Hinge). The exact sequence is proprietary.

  • Positive control
    • Human spleen and Human plasma lysates; Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP4420
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Relevance
      IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Ig gamma 4 chain C region antibody
      • IGHG4 antibody
      • immunoglobulin heavy chain constant region gamma 4 antibody
      • Immunoglobulin heavy constant gamma 4 (G4m marker) antibody
      • MGC117419 antibody
      see all

    Images

    • All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution

      Lane 1 : IgG4 Recombinant protein at 0.005 µg
      Lane 2 : IgG1 Recombinant protein at 0.01 µg
      Lane 3 : IgG2 Recombinant protein at 0.01 µg
      Lane 4 : IgG3 Recombinant protein at 0.01 µg
      Lane 5 : Human spleen lysate at 10 µg
      Lane 6 : Human plasma lysate at 10 µg

      Predicted band size: 36 kDa

    • ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.

    References

    This product has been referenced in:
    • Zhao PX  et al. IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease. Int J Ophthalmol 11:36-42 (2018). Read more (PubMed: 29375988) »
    • Jing Z  et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). Read more (PubMed: 29512722) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    I have reviewed these products and have been in touch with the labs regarding reactivity with Rhesus as requested. We would not recommend any human IgG2 antibody which we know is directed to the hinge region, such as ab1935, against Rhesus as the homology of these is poor. However with this exception, in general there seems to be good homology between Rhesus and Human IgG subtypes. More information about human and Rhesus IgGs may be found at the links below: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1782391/ http://nhpreagents.bidmc.harvard.edu/nhp/macaqueisotypes.aspx http://www.kcl.ac.uk/immunobiology/Mac_ig/ We have not tested any of these products against Rhesus. For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial We could also offer enrollment in the AbTrial program for anti-IgG4 ab109493. We do not currently have any anti-IgG3 primary antibodies. More information about ab109493 can be found at the locations below: Rabbit monoclonal [EP4420] to IgG4: https://www.abcam.com/igg4-antibody-ep4420-ab109493.html

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    Answer

    Gracias por tu respuesta.
    Este es un mail de confirmación del nuevo pedido, consistente en el reemplazo completamente gratuito, del anticuerpo ab99466 y el envío de ab109493 como parte de la promoción para testar anticuerpos RabMab. El número de pedido es XXXXX.
    Te llegará un mail de confirmación del nuevo pedido, si necesitas información sobre el estado del mismo no dudes en contactarnos haciendo referencia al número indicado.
    Espero que este nuevo anticuerpo funcione según lo esperado. En caso contrario, te recuerdo que estos anticuerpos también tienen una garantía de 6 meses.
    Que pases un buen día.

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    Question
    Answer

    Thank you for contacting Abcam.

    This product is expected to remain stable for up to 1 year from receipt when stored properly at -20oC.

    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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