Product nameAnti-IgG4 antibody [EP4420]
See all IgG4 primary antibodies
DescriptionRabbit monoclonal [EP4420] to IgG4
SpecificityDoes not cross-react with IgG1, IgG2, or IgG3.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human IgG4 aa 50-150 (Hinge). The exact sequence is proprietary.
- Human spleen and Human plasma lysates; Human tonsil tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
RelevanceIgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
- Ig gamma 4 chain C region antibody
- IGHG4 antibody
- immunoglobulin heavy chain constant region gamma 4 antibody
All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution
Lane 1 : IgG4 Recombinant protein at 0.005 µg
Lane 2 : IgG1 Recombinant protein at 0.01 µg
Lane 3 : IgG2 Recombinant protein at 0.01 µg
Lane 4 : IgG3 Recombinant protein at 0.01 µg
Lane 5 : Human spleen lysate at 10 µg
Lane 6 : Human plasma lysate at 10 µg
Predicted band size: 36 kDa
ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.
This product has been referenced in:
- Zhao PX et al. IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease. Int J Ophthalmol 11:36-42 (2018). Read more (PubMed: 29375988) »
- Jing Z et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). Read more (PubMed: 29512722) »