Recombinant Anti-IgG4 antibody [EP4420] (ab109493)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4420] to IgG4
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
-
Product name
Anti-IgG4 antibody [EP4420]
See all IgG4 primary antibodies -
Description
Rabbit monoclonal [EP4420] to IgG4 -
Host species
Rabbit -
Specificity
Does not cross-react with IgG1, IgG2, or IgG3. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human IgG4 recombinant protein; Human spleen lysate, Human plasma lysate IHC-P: Human tonsil tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4420 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 36 kDa.
For unpurified, use at 1/1000 - 1/10000. |
|
IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 36 kDa. For unpurified, use at 1/1000 - 1/10000. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Relevance
IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 3503 Human
- Omim: 147130 Human
- SwissProt: P01861 Human
-
Alternative names
- Ig gamma 4 chain C region antibody
- IGHG4 antibody
- immunoglobulin heavy chain constant region gamma 4 antibody
see all
Images
-
All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution (Purified)
Lane 1 : Human spleen lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG4 with Purified ab109493 at 1:4000 dilution (0.023 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
-
All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution
Lane 1 : IgG4 Recombinant protein at 0.005 µg
Lane 2 : IgG1 Recombinant protein at 0.01 µg
Lane 3 : IgG2 Recombinant protein at 0.01 µg
Lane 4 : IgG3 Recombinant protein at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab109493 has been referenced in 17 publications.
- Deng Z et al. Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33760139
- Fernández de Larrea C et al. Defining an Optimal Dual-Targeted CAR T-cell Therapy Approach Simultaneously Targeting BCMA and GPRC5D to Prevent BCMA Escape-Driven Relapse in Multiple Myeloma. Blood Cancer Discov 1:146-154 (2020). PubMed: 33089218
- Della-Torre E et al. B lymphocytes directly contribute to tissue fibrosis in patients with IgG4-related disease. J Allergy Clin Immunol 145:968-981.e14 (2020). PubMed: 31319101
- Maritati F et al. Clinical and Prognostic Significance of Serum IgG4 in Chronic Periaortitis. An Analysis of 113 Patients. Front Immunol 10:693 (2019). PubMed: 31019511
- Lv X et al. Clinical and pathological characteristics of IgG4-related interstitial lung disease. Exp Ther Med 15:1465-1473 (2018). PubMed: 29434730