Product nameAnti-IgG4 antibody [EP4420] - BSA and Azide free
See all IgG4 primary antibodies
DescriptionRabbit monoclonal [EP4420] to IgG4 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human IgG4 aa 50-150 (Hinge). The exact sequence is proprietary.
- Human spleen and Human plasma lysates; Human tonsil tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This antibody is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab271883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceIgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
- Ig gamma 4 chain C region antibody
- IGHG4 antibody
- immunoglobulin heavy chain constant region gamma 4 antibody
ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109493).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271883 has not yet been referenced specifically in any publications.