Product nameAnti-IgG4 antibody [EP4420] - Low endotoxin, Azide free
See all IgG4 primary antibodies
DescriptionRabbit monoclonal [EP4420] to IgG4 - Low endotoxin, Azide free
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IgG4 aa 50-150 (Hinge). The exact sequence is proprietary.
Database link: P01861
- IHC-P: Human tonsil tissue.
ab186921 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186921 in the following tested applications.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.|
RelevanceIgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
- Ig gamma 4 chain C region antibody
- IGHG4 antibody
- immunoglobulin heavy chain constant region gamma 4 antibody
This IHC data was generated using the same anti-IgG4 antibody clone, EP4420, in a different buffer formulation (cat# ab109493).
ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
ab186921 has been referenced in 1 publication.
- London J et al. Adult Onset Asthma and Periocular Xanthogranuloma (AAPOX), a Rare Entity With a Strong Link to IgG4-Related Disease: An Observational Case Report Study. Medicine (Baltimore) 94:e1916 (2015). IHC . PubMed: 26512617