Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM120] to IgG4
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Product nameAnti-IgG4 antibody [RM120]
See all IgG4 primary antibodies
DescriptionRabbit monoclonal [RM120] to IgG4
ab238320 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human IgG4 (Hinge).
Database link: P01861
- WB: Reduced and non-reduced human IgG4. IHC-P: Human lymphoid tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesProtein A affinity purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 36 kDa.|
|IHC-P||Use a concentration of 1 - 10 µg/ml.|
|ELISA||Use a concentration of 0.05 - 0.2 µg/ml.|
RelevanceIgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
- Ig gamma 4 chain C region antibody
- IGHG4 antibody
- immunoglobulin heavy chain constant region gamma 4 antibody
All lanes : Anti-IgG4 antibody [RM120] (ab238320) at 2 µg/ml
Lane 1 : non-reduced (-) and reduced (+) forms of Human IgG1
Lane 2 : non-reduced (-) and reduced (+) forms of Human IgG2
Lane 3 : non-reduced (-) and reduced (+) forms of Human IgG3
Lane 4 : non-reduced (-) and reduced (+) forms of Human IgG4
Lane 5 : non-reduced (-) and reduced (+) forms of Mouse IgG
Lane 6 : non-reduced (-) and reduced (+) forms of rat IgG
Lane 7 : non-reduced (-) and reduced (+) forms of goat IgG
Predicted band size: 36 kDa
The Western blot shows ab238320 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG is shown.
Formalin-fixed, Paraffin-embedded human lymphoid tissue stained for Human IgG4 using ab238320 at 2 μg/mL in immunohistochemical analysis.
Sandwich ELISA using ab238320 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
A titer ELISA using ab238320. The plate was coated with different amounts of human IgG4. A serial dilution of ab238320 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Detection of human IgG4 in monkey serum. Sandwich ELISA using ab238320 as the capture antibody, and a biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by a HRP conjugated streptavidin.
ELISA showing ab238320 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of ab238320 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238320 has not yet been referenced specifically in any publications.