Recombinant
RabMAb

Recombinant Anti-IGJ antibody [SP105] (ab105229)

Overview

  • Product name

    Anti-IGJ antibody [SP105]
    See all IGJ primary antibodies
  • Description

    Rabbit monoclonal [SP105] to IGJ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human IGJ aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P01591

  • Positive control

    • IHC-P: Human tonsil and stomach tissue FC: Daudi cells
  • General notes

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP105
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
IHC-P 1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function

    Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • IGCJ antibody
    • IGJ_HUMAN antibody
    • Immunoglobulin J chain antibody
    • Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody
    • immunoglobulin joining chain antibody
    • JCH antibody
    • JCHAIN antibody
    • Joining chain of multimeric IgA and IgM antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab105229 for 10 mins at room temperature.

  • Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab105229 for 10 mins at room temperature.

  • ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

References

ab105229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your call yesterday and for letting us know about the trouble with ab105229.

I've heard back from the lab, and we have not received other reports of the antibody not working, so it's possible that the protocol can be altered to produce better results. The only alterations that we would suggestare to increasethe antigen retrieval and primary antibody incubation times. We also have not tried the antibody with colon and appendix sections, so if you have any tonsil slides we would suggest using them as a positive control. You may also want to try an antibody that you've previously optimized on the colon or appendix tissue, just to make sure the tissue is not too old.

As we discussed, if the staining results do not improve, I would be happy to send a different IGJ antibody free of charge.

Please keep me updated about any progress, and let me know if you have any questions or need anything else from me.

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