Recombinant

Recombinant Anti-IGLC1 antibody [RM127] (Biotin) (ab224180)

Overview

  • Product name

    Anti-IGLC1 antibody [RM127] (Biotin)
  • Description

    Rabbit monoclonal [RM127] to IGLC1 (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    ab224180 reacts to the lambda light chain of human immunoglobulins.
  • Tested applications

    Suitable for: ELISA, IHCmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Goat
  • Immunogen

    Full length native protein (purified) corresponding to Human IGLC1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab224180 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM127
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.05 - 0.2 µg/ml.
IHC Use a concentration of 0.5 - 2 µg/ml.

Target

  • Cellular localization

    Extracellular region, plasma membrane.
  • Database links

  • Alternative names

    • Ig lambda-1 chain C regions antibody
    • IGLC antibody
    • immunoglobulin lambda constant 1 (Mcg marker) antibody
    • Immunoglobulin lambda constant region 1 antibody
    • Immunoglobulin: lambda light chain antibody
    see all

Images

  • A titer ELISA using ab224180. The plate was coated with different amounts of human IgG3λ. A serial dilution of ab224180 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.

  • ELISA of human immunoglobulins shows ab224180 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of ab224180 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.

  • Human tonsil tissue stained for IGLC1 using ab224180 at 2 µg/ml in immunohistochemical analysis.

References

ab224180 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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