Key features and details
- Mouse monoclonal [IM260 + IM373 + ICO-30] to IgM
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IgM antibody [IM260 + IM373 + ICO-30]
See all IgM primary antibodies
DescriptionMouse monoclonal [IM260 + IM373 + ICO-30] to IgM
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IgM. Recombinant heavy chain of human IgM (IM260 & IM373); Heavy chain of human IgM (ICO-30).
- IHC-P; Human tonsil tissue.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Antibody isotypes: Mouse IgG1 and IgG2b kappa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesab233887 is purified from bioreactor concentrate by Protein A/G.
Clone numberIM260 + IM373 + ICO-30
Light chain typekappa
Our Abpromise guarantee covers the use of ab233887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceIgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
Cellular localizationIsoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
- DKFZp686I15196 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233887 has not yet been referenced specifically in any publications.