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Immunology Immunoglobulins Heavy Chain IgM
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Anti-IgM antibody, prediluted (ab75669)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgM antibody, prediluted (ab75669)

    Key features and details

    • Rabbit polyclonal to IgM, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human IgM protein (Tagged) (ab158748)
    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-IgM antibody, prediluted
      See all IgM primary antibodies
    • Description

      Rabbit polyclonal to IgM, prediluted
    • Host species

      Rabbit
    • Specificity

      This antibody recognizes the mu heavy chain of human immunoglobulins. It does not cross-react with gamma (IgG), alpha (IgA), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length human IgM protein purified from serum of a patient with Waldenstrom's Macroglobulinaemia.

    • Positive control

      • Human tonsil tissue.
    • General notes


      This antibody can be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.60
      Preservative: 0.097% Sodium azide
      Constituent: 7.9% Tris HCl

      Containing antibody stabilizer
    • Concentration information loading...
    • Purity

      Protein A purified
    • Primary antibody notes

      This antibody can be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgM
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human IgM protein (Tagged) (ab158748)

    Applications

    Our Abpromise guarantee covers the use of ab75669 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Ready to use.

    Target

    • Relevance

      IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
    • Cellular localization

      Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
    • Database links

      • Entrez Gene: 3507 Human
      • Omim: 147020 Human
      • SwissProt: P01871 Human
      • Alternative names

        • AGM1 antibody
        • Constant region of heavy chain of IgM antibody
        • DKFZp686I15196 antibody
        • DKFZp686I15212 antibody
        • FLJ00385 antibody
        • Hepatitis B virus receptor binding protein antibody
        • Ig mu chain C region antibody
        • IGHM antibody
        • IgM heavy chain constant region antibody
        • Immunoglobulin heavy constant mu antibody
        • Immunoglobulin mu chain antibody
        • Imunoglobulin heavy chain antibody
        • Imunoglobulin heavy chain constant region mu antibody
        • Imunoglobulin heavy chain mu constant region antibody
        • MGC104996 antibody
        • MGC52291 antibody
        • MU antibody
        • VH antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgM antibody, prediluted (ab75669)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgM antibody, prediluted (ab75669)
        Formalin-fixed, paraffin-embedded human tonsil stained with ab75669, using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgM-secreting cells.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab75669? Please let us know so that we can cite the reference in this datasheet.

      ab75669 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      What is the concentration of the lot I'm using? For this antibody, what is the dilution factor from the undiluted version (1:100, etc)?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 15 2012

      Answer

      Thank you for your call yesterday and for your patience while I have been in touch with the lab.

      My colleague has told me that the equivalent dilution factor would be between 1:500 and 1:1000, and the exact concentration is not available for this antibody.

      I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

      Read More

      Abcam Scientific Support

      Answered on Jun 15 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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