Anti-IgM antibody, prediluted (ab75669)
Key features and details
- Rabbit polyclonal to IgM, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IgM antibody, prediluted
See all IgM primary antibodies -
Description
Rabbit polyclonal to IgM, prediluted -
Host species
Rabbit -
Specificity
This antibody recognizes the mu heavy chain of human immunoglobulins. It does not cross-react with gamma (IgG), alpha (IgA), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length human IgM protein purified from serum of a patient with Waldenstrom's Macroglobulinaemia.
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Positive control
- Human tonsil tissue.
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General notes
This antibody can be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.097% Sodium azide
Constituent: 7.9% Tris HCl
Containing antibody stabilizer -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody can be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab75669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Ready to use. |
Target
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Relevance
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated. -
Cellular localization
Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein. -
Database links
- Entrez Gene: 3507 Human
- Omim: 147020 Human
- SwissProt: P01871 Human
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Alternative names
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
- DKFZp686I15196 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgM antibody, prediluted (ab75669)Formalin-fixed, paraffin-embedded human tonsil stained with ab75669, using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgM-secreting cells.
References (0)
ab75669 has not yet been referenced specifically in any publications.
