Key features and details
- Mouse monoclonal [UCH-B1] to IgM
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IgM antibody [UCH-B1]
See all IgM primary antibodies
DescriptionMouse monoclonal [UCH-B1] to IgM
Tested applicationsSuitable for: ICC/IF, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Human prolymphocytic leukaemia B cells.
UCH-B1 antigen (85kD) is detected on a sub-population of B lymphocytes, probably related to stage of activation or maturation. UCH-B1 may be useful for differential diagnosis of B cell leukaemias and as a marker of prolymphocytic leukaemia.
Does not work on formalin fixed and parrafin embedded tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Primary antibody notesUCH-B1 antigen (85kD) is detected on a sub-population of B lymphocytes, probably related to stage of activation or maturation. UCH-B1 may be useful for differential diagnosis of B cell leukaemias and as a marker of prolymphocytic leukaemia.
Our Abpromise guarantee covers the use of ab18201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceIgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
Cellular localizationIsoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
- DKFZp686I15196 antibody
ab18201 has been referenced in 4 publications.
- Grupp SA et al. Molecular mechanisms that control expression of the B lymphocyte antigen receptor complex. J Exp Med 181:161-8 (1995). PubMed: 7807000
- DeFranco AL et al. Structure and function of the B-cell antigen receptor. Chem Immunol 59:156-72 (1994). PubMed: 7945925
- Smith-Ravin J et al. Characterization of two monoclonal antibodies (UCL4D12 and UCL3D3) that discriminate between human mantle zone and marginal zone B cells. Clin Exp Immunol 82:181-7 (1990). PubMed: 2208792
- Armitage RJ et al. A new antigen identified by the monoclonal antibody UCHB 1 delivers a costimulatory signal to a subset of human B cells. Eur J Immunol 18:67-76 (1988). PubMed: 3257923