Key features and details
- Rabbit polyclonal to IGSF1/p120
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IGSF1/p120 antibody
See all IGSF1/p120 primary antibodies
DescriptionRabbit polyclonal to IGSF1/p120
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog
- WB: HepG2 and 293T cell lysates. IHC-P: Human kidney tissue.
Product previously known as Anti-IGSF1 antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionSeems to be a coreceptor in inhibin signaling, but seems not to be a high-affinity inhibin receptor. Antagonizes activin A signaling in the presence or absence of inhibin B (By similarity). Necessary to mediate a specific antagonistic effect of inhibin B on activin-stimulated transcription.
Tissue specificityHighly expressed in pancreas, testis and fetal liver. Moderately expressed in heart, prostate and small intestine. Expressed at very low levels in brain, thymus, ovary, colon, fetal lung and fetal kidney. Expressed in muscle. Isoform 3 is expressed in pituitary gland.
Involvement in diseaseHypothyroidism, central, and testicular enlargement
Sequence similaritiesContains 12 Ig-like C2-type (immunoglobulin-like) domains.
Developmental stageExpressed in embryo Carnegie stage 18 in Rathke's pouch progenitors.
Cellular localizationSecreted and Membrane.
- Information by UniProt
- IGCD1 antibody
- IGDC1 antibody
- IgSF1 antibody
Anti-IGSF1/p120 antibody (ab66509) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IGSF1/p120 with ab66509 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
All lanes : Anti-IGSF1/p120 antibody (ab66509) at 3 µg/ml
Lane 1 : 293T cell lysate with Milk
Lane 2 : Human liver tumor lysate with Milk
Lysates/proteins at 25 µg per lane.
Blocking peptides at 5 % per lane.
Predicted band size: 27 kDa
Western blot analysis labelling IGSF1 with ab66509.
ab66509 has been referenced in 1 publication.
- Faucz FR et al. Is IGSF1 involved in human pituitary tumor formation? Endocr Relat Cancer 22:47-54 (2015). PubMed: 25527509