• Product name
  • Description
    Rabbit polyclonal to IGSF8
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human IGSF8.

    (Peptide available as ab74889.)

  • Positive control
    • This antibody gave a positive signal in Human Brain Tissue Lysate.



Our Abpromise guarantee covers the use of ab74890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).


  • Function
    May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
  • Tissue specificity
    Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.
  • Sequence similarities
    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain
    The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.
    The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD316 antibody
    • CD316 antigen antibody
    • CD81 partner 3 antibody
    • CD81P3 antibody
    • EWI-2 antibody
    • EWI2 antibody
    • Glu-Trp-Ile EWI motif-containing protein 2 antibody
    • igsf-8 antibody
    • IgSF8 antibody
    • IGSF8_HUMAN antibody
    • Immunoglobulin superfamily member 8 antibody
    • Immunoglobulin superfamily member 8 precursor antibody
    • KCT-4 antibody
    • KCT4 antibody
    • Keratinocytes-associated transmembrane protein 4 antibody
    • LIR-D1 antibody
    • PGRL antibody
    • Prostaglandin regulatory-like protein antibody
    see all


  • Anti-IGSF8 antibody (ab74890) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

    Exposure time: 20 minutes

    IGSF8 contains a number of potential glycosylation sites and a phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.


This product has been referenced in:
  • Liang Y  et al. Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J Biol Chem 289:32526-37 (2014). Read more (PubMed: 25261472) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74890.
Please use the links above to contact us or submit feedback about this product.


Sign up