Recombinant
RabMAb

Recombinant Anti-Ihh antibody [EP1192Y] (ab52919)

Reviews (1)Q&A (2)References (16)

Overview

  • Product name

    Anti-Ihh antibody [EP1192Y]
    See all Ihh primary antibodies

  • Description

    Rabbit monoclonal [EP1192Y] to Ihh

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-FoFrmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human IHH.

  • Positive control

    • Human fetal colon tissue, fetal brain lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22558278
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

    • Tissue specificity

      Expressed in embryonic lung, and in adult kidney and liver.

    • Involvement in disease

      Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short.
      Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles.

    • Sequence similarities

      Belongs to the hedgehog family.

    • Post-translational
      modifications

      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
      Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
      Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.

    • Cellular localization

      Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BDA1 antibody
      • HHG-2 antibody
      • HHG2 antibody
      • IHH antibody
      • IHH_HUMAN antibody
      • Indian Hedgehog antibody
      • Indian hedgehog homolog antibody
      • Indian hedgehog protein antibody
      • Indian hedgehog protein C-product antibody
      • Indian hedgehog protein N-product antibody
      see all

    Images

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] (ab52919)Image from Dahia CL et al., PLoS One. 2012;7(4):e35944. Epub 2012 Apr 27. Fig 5.; doi:10.1371/journal.pone.0035944; April 27, 2012, PLoS ONE 7(4): e35944.
      Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with ab52919.

      Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5®-conjugated goat anti-rabbit IgG as secondary antibody.
    • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution + Human fetal brain lysate at 10 µg

      Secondary
      Goat anti rabbit hrp conjugated at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
      Ab52919 at 1/250 dilution staining human fetal colon tissue; paraffin embedded.

    References

    This product has been referenced in:

    • Fu X  et al. Induced lesion and inhibited Ihh-PTHrP signalling pathway activity in the articular cartilage of rats caused by T-2 toxin. Toxicon 158:104-108 (2019). Read more (PubMed: 30550741) »
    • Guo Y  et al. BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair. Bone Res 6:30 (2018). Read more (PubMed: 30345151) »
    See all 16 Publications for this product

    Publishing research using ab52919? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Phone call requesting information on if the Anti-Ihh antibody [EP1192Y] (ab52919) is likely to cross react with the protein derived from bovine samples.

    Read More
    Answer

    I have tried to check the likelihood of the Anti-Ihh antibody [EP1192Y] (ab52919) being able to cross-react with the protein from bovine samples. Unfortunately, the only protein sequence I have been able to find for the bovine Ihh protein is of the Uniprot reference http://www.uniprot.org/uniprot/Q08D77. This seems to be N-truncated, or be naturally shorter than the human and mouse protein. As the antibody ab52919 has been raised against a peptide derived from the N-terminal region, I cannot say whether it would be able to cross-react or not. From the sequence I have access to it seems it may not.

    However, we do have an alternative antibody which may work better for you. This is the Rabbit polyclonal to Ihh, ab39634. This has been raised against a peptide taken from the C-terminus of the mouse protein, which shows high homology with the bovine sequence (please refer to the sequence alignment attached) and I would therefore expect it to cross react. However, as we have not tested this ourselves I would not be able to guarantee this. If you would be interested, you would qualify for our Abtrial scheme. More information on this offer can be found here:

    www.abcam.com/abtrial

    Read More

    Question

    Vielen dank,
    nun habe ich Ihre mail erhalten und die Fragen beantwortet,ich sende sie
    anbei zurück und bitte Sie herzlich um rasche Bearbeitung und Antwort.
    Vielen dank
    Herzliche Grüße

    Read More
    Answer

    Ich habe Ihre Ergebnisse an das Labor, das diesen Antikoerper herstellt, weitergeleitet.
    Es wird nun nachgeforscht, wo das Problem liegt.
    Da ich nicht moechte,dass Sie so lange warten muessen, wuerde ich Ihnen gernen einen alternativen Antikoerper als Ersatz anbieten. Oder auch eine Gutschrift, wenn Sie das bevorzugen.
    Es tut mir sehr leid, dass dieser Antikoeprer nicht den Beschreibungen auf dem Datenblatt entsprochen hat. Ich moechte mich auch dafuer bedanken, dass Sie uns auf dieses Problem aufmerksam gemacht haben.
    Bitte lassen Sie mich wissen, welchen Antikoerper Sie gerne als Ersatz haetten.
    Ich bin gerade in unserer amerikanischen Niederlassung: Bitte entschuldigen Sie die fehlenden Umlaute.
    Ich freue mich auf Ihre Antwort und verbleibe

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-Ihh antibody [EP1192Y]

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Chondrocytes)
    Specification
    Chondrocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
    Read More

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    Submitted Jan 27 2011

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