Recombinant
RabMAb

Recombinant Anti-Ihh antibody [EP1192Y] (ab52919)

Reviews (1)Q&A (2)References (16)

Overview

  • Product name

    Anti-Ihh antibody [EP1192Y]
    See all Ihh primary antibodies

  • Description

    Rabbit monoclonal [EP1192Y] to Ihh

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-FoFrmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human Ihh aa 1-100.

  • Positive control

    • WB: Mouse brain, Rat brain, Human fetal colon tissue, fetal brain lysate and Human brain lysates. IHC-P: human liver, mouse liver, and rat liver tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1192Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 45 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22558278

Target

  • Function

    Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

  • Tissue specificity

    Expressed in embryonic lung, and in adult kidney and liver.

  • Involvement in disease

    Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short.
    Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles.

  • Sequence similarities

    Belongs to the hedgehog family.

  • Post-translational
    modifications

    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.

  • Cellular localization

    Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BDA1 antibody
    • HHG-2 antibody
    • HHG2 antibody
    • IHH antibody
    • IHH_HUMAN antibody
    • Indian Hedgehog antibody
    • Indian hedgehog homolog antibody
    • Indian hedgehog protein antibody
    • Indian hedgehog protein C-product antibody
    • Indian hedgehog protein N-product antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Ihh with Purified ab52919 at 1:50 dilution (3.16 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Ihh with Purified ab52919 at 1:50 dilution (3.16 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Ihh with Purified ab52919 at 1:50 dilution (3.16 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
    Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
    Anti-Ihh antibody [EP1192Y] (ab52919) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
    Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
    All lanes : Anti-Ihh antibody [EP1192Y] (ab52919) at 1/1000 dilution (Purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] (ab52919)Image from Dahia CL et al., PLoS One. 2012;7(4):e35944. Epub 2012 Apr 27. Fig 5.; doi:10.1371/journal.pone.0035944; April 27, 2012, PLoS ONE 7(4): e35944.

    Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with unpurified ab52919.

    Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5®-conjugated goat anti-rabbit IgG as secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)

    ab52919 (unpurified) at 1/250 dilution staining human fetal colon tissue; paraffin embedded.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Fu X  et al. Induced lesion and inhibited Ihh-PTHrP signalling pathway activity in the articular cartilage of rats caused by T-2 toxin. Toxicon 158:104-108 (2019). Read more (PubMed: 30550741) »
  • Guo Y  et al. BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair. Bone Res 6:30 (2018). Read more (PubMed: 30345151) »
See all 16 Publications for this product

Publishing research using ab52919? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Phone call requesting information on if the Anti-Ihh antibody [EP1192Y] (ab52919) is likely to cross react with the protein derived from bovine samples.

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Answer

I have tried to check the likelihood of the Anti-Ihh antibody [EP1192Y] (ab52919) being able to cross-react with the protein from bovine samples. Unfortunately, the only protein sequence I have been able to find for the bovine Ihh protein is of the Uniprot reference http://www.uniprot.org/uniprot/Q08D77. This seems to be N-truncated, or be naturally shorter than the human and mouse protein. As the antibody ab52919 has been raised against a peptide derived from the N-terminal region, I cannot say whether it would be able to cross-react or not. From the sequence I have access to it seems it may not.

However, we do have an alternative antibody which may work better for you. This is the Rabbit polyclonal to Ihh, ab39634. This has been raised against a peptide taken from the C-terminus of the mouse protein, which shows high homology with the bovine sequence (please refer to the sequence alignment attached) and I would therefore expect it to cross react. However, as we have not tested this ourselves I would not be able to guarantee this. If you would be interested, you would qualify for our Abtrial scheme. More information on this offer can be found here:

www.abcam.com/abtrial

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Question

Vielen dank,
nun habe ich Ihre mail erhalten und die Fragen beantwortet,ich sende sie
anbei zurück und bitte Sie herzlich um rasche Bearbeitung und Antwort.
Vielen dank
Herzliche Grüße

Read More
Answer

Ich habe Ihre Ergebnisse an das Labor, das diesen Antikoerper herstellt, weitergeleitet.
Es wird nun nachgeforscht, wo das Problem liegt.
Da ich nicht moechte,dass Sie so lange warten muessen, wuerde ich Ihnen gernen einen alternativen Antikoerper als Ersatz anbieten. Oder auch eine Gutschrift, wenn Sie das bevorzugen.
Es tut mir sehr leid, dass dieser Antikoeprer nicht den Beschreibungen auf dem Datenblatt entsprochen hat. Ich moechte mich auch dafuer bedanken, dass Sie uns auf dieses Problem aufmerksam gemacht haben.
Bitte lassen Sie mich wissen, welchen Antikoerper Sie gerne als Ersatz haetten.
Ich bin gerade in unserer amerikanischen Niederlassung: Bitte entschuldigen Sie die fehlenden Umlaute.
Ich freue mich auf Ihre Antwort und verbleibe

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Immunohistochemistry (Frozen sections) abreview for Anti-Ihh antibody [EP1192Y]

 Below Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Chondrocytes)
Specification
Chondrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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Submitted Jan 27 2011

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