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Phone call requesting information on if the Anti-Ihh antibody [EP1192Y] (ab52919) is likely to cross react with the protein derived from bovine samples.
Asked on Feb 19 2013
I have tried to check the likelihood of the Anti-Ihh antibody [EP1192Y] (ab52919) being able to cross-react with the protein from bovine samples. Unfortunately, the only protein sequence I have been able to find for the bovine Ihh protein is of the Uniprot reference http://www.uniprot.org/uniprot/Q08D77. This seems to be N-truncated, or be naturally shorter than the human and mouse protein. As the antibody ab52919 has been raised against a peptide derived from the N-terminal region, I cannot say whether it would be able to cross-react or not. From the sequence I have access to it seems it may not.
However, we do have an alternative antibody which may work better for you. This is the Rabbit polyclonal to Ihh, ab39634. This has been raised against a peptide taken from the C-terminus of the mouse protein, which shows high homology with the bovine sequence (please refer to the sequence alignment attached) and I would therefore expect it to cross react. However, as we have not tested this ourselves I would not be able to guarantee this. If you would be interested, you would qualify for our Abtrial scheme. More information on this offer can be found here:
Answered on Feb 19 2013