Recombinant
RabMAb

Recombinant Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)

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Overview

  • Product name

    Anti-Ihh antibody [EP1192Y] - BSA and Azide free
    See all Ihh primary antibodies

  • Description

    Rabbit monoclonal [EP1192Y] to Ihh - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IHC-FoFrmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ihh aa 1-100. The exact sequence is proprietary.

  • Positive control

    • IHC-P: human liver, mouse liver, and rat liver tissues.

  • General notes

    Ab239853 is the carrier-free version of ab52919. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239853 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab239853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22558278

Target

  • Function

    Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

  • Tissue specificity

    Expressed in embryonic lung, and in adult kidney and liver.

  • Involvement in disease

    Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short.
    Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles.

  • Sequence similarities

    Belongs to the hedgehog family.

  • Post-translational
    modifications

    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.

  • Cellular localization

    Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BDA1 antibody
    • HHG-2 antibody
    • HHG2 antibody
    • IHH antibody
    • IHH_HUMAN antibody
    • Indian Hedgehog antibody
    • Indian hedgehog homolog antibody
    • Indian hedgehog protein antibody
    • Indian hedgehog protein C-product antibody
    • Indian hedgehog protein N-product antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Ihh with Purified ab52919 at 1:50 dilution (3.16 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Ihh with Purified ab52919 at 1:50 dilution (3.16 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Ihh with Purified ab52919 at 1:50 dilution (3.16 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)

    ab52919 (unpurified) at 1/250 dilution staining human fetal colon tissue; paraffin embedded.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y] - BSA and Azide free (ab239853)Image from Dahia CL et al. PLoS One. 2012;7(4):e35944. Epub 2012 Apr 27. Fig 5.; doi:10.1371/journal.pone.0035944; April 27 2012 PLoS ONE 7(4): e35944.

    Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with unpurified ab52919.

    Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5®-conjugated goat anti-rabbit IgG as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52919).

References

ab239853 has not yet been referenced specifically in any publications.

Publishing research using ab239853? Please let us know so that we can cite the reference in this datasheet.

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