Key features and details
- Rabbit polyclonal to IKAP/p150
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IKAP/p150 antibody
See all IKAP/p150 primary antibodies
DescriptionRabbit polyclonal to IKAP/p150
This antibody reacts with IKAP/p150
Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human IKAP/p150.
- A20 cell lysate and A20 cells
This product was previously labelled as IKAP
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa).|
|ICC||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionMay act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Involvement in diseaseDefects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
Sequence similaritiesBelongs to the ELP1/IKA1 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DKFZp781H1425 antibody
- DYS antibody
- Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
Lane 1 : Anti-IKAP/p150 antibody (ab62498) at 0.5 µg/ml
Lane 2 : Anti-IKAP/p150 antibody (ab62498) at 1 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG
Predicted band size: 148 kDa
Observed band size: 100,148 kDa why is the actual band size different from the predicted?
Immunocytochemistry of IKAP/p150 in A20 cells with ab62498 antibody at 1 µg/ml.
Immunofluorescence of IKAP/p150 in A20 cells using ab62498 at 20 ug/ml.
ab62498 has not yet been referenced specifically in any publications.