Product nameAnti-IKAP/p150 antibody [EPR13113]
See all IKAP/p150 primary antibodies
DescriptionRabbit monoclonal [EPR13113] to IKAP/p150
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment within Human IKAP/p150. The exact sequence is proprietary.
Database link: O95163
- HepG2, HeLa, Jurkat and 293T cell lysates; HeLa cells; Jurkat cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as IKAP
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab179437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 150 kDa.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Involvement in diseaseDefects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
Sequence similaritiesBelongs to the ELP1/IKA1 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DKFZp781H1425 antibody
- DYS antibody
- Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
All lanes : Anti-IKAP/p150 antibody [EPR13113] (ab179437) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 150 kDa
Immunofluorescent analysis of HeLa cells labeling IKAP/p150 with ab179437 at 1/50 dilution (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized Jurkat cells labeling IKAP/p150 with ab179437 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).