Recombinant
RabMAb

Recombinant Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)

Overview

  • Product name

    Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free
    See all IKAP/p150 primary antibodies
  • Description

    Rabbit monoclonal [EPR13113] to IKAP/p150 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IKAP/p150. The exact sequence is proprietary.
    Database link: O95163

  • Positive control

    • HepG2, HeLa, Jurkat and 293T cell lysates; HeLa cells; Jurkat cells.
  • General notes

    ab210396 is the carrier-free version of ab179437 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab210396 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as IKAP

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 150 kDa.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
      Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
    • Involvement in disease

      Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
    • Sequence similarities

      Belongs to the ELP1/IKA1 family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp781H1425 antibody
      • DYS antibody
      • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
      • Elongator complex protein 1 antibody
      • ELP 1 antibody
      • ELP1 antibody
      • ELP1_HUMAN antibody
      • FD antibody
      • FLJ12497 antibody
      • IKAP antibody
      • IkappaB kinase complex associated protein antibody
      • IkappaB kinase complex-associated protein antibody
      • ikbkap antibody
      • IKI 3 antibody
      • IKI3 antibody
      • IKK complex associated protein antibody
      • IKK complex-associated protein antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
      • OTTHUMP00000063889 antibody
      • p150 antibody
      • TOT 1 antibody
      • TOT1 antibody
      see all

    References

    ab210396 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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