Overview

  • Product name

  • Description

    Mouse polyclonal to Ikaros
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Full length protein, corresponding to amino acids 1-477 of Human Ikaros Isoform Ik7 (AAH18349.1, UniProt ID: Q13422-7).

  • Positive control

    • Recombinant Human Ikaros protein (ab132325) can be used as a positive control in WB. Lysate from 293T cells transfected with Ikaros.

Properties

Applications

Our Abpromise guarantee covers the use of ab168757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.

Target

  • Function

    Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
  • Tissue specificity

    Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
  • Involvement in disease

    Defects in IKZF1 are frequent occurrences (28.6%) in acute lymphoblasic leukemia (ALL). Such alterations or deletions lead to poor prognosis for ALL.
    Chromosomal aberrations involving IKZF1 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;7)(q27;p12), with BCL6.
  • Sequence similarities

    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Domain

    The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin.
    The C-terminal zinc-finger domain is required for dimerization.
  • Post-translational
    modifications

    Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
    Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1.
    Polyubiquitinated.
  • Cellular localization

    Cytoplasm; Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events and Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity).
  • Information by UniProt
  • Database links

  • Form

    There are 7 isoforms produced by alternative splicing.
  • Alternative names

    • CLL associated antigen KW 6 antibody
    • DNA-binding protein Ikaros antibody
    • hIk 1 antibody
    • Hs.54452 antibody
    • IK1 antibody
    • Ikaros (zinc finger protein) antibody
    • IKAROS antibody
    • IKAROS family zinc finger 1 (Ikaros) antibody
    • Ikaros family zinc finger protein 1 antibody
    • Ikzf1 antibody
    • IKZF1_HUMAN antibody
    • LYF1 antibody
    • Lymphoid transcription factor LyF-1 antibody
    • PRO0758 antibody
    • Zinc finger protein subfamily 1A 1 (Ikaros) antibody
    • Zinc finger protein subfamily 1A 1 antibody
    • Zinc finger protein, subfamily 1A, member 1 antibody
    • ZNFN1A1 antibody
    see all

Images

  • All lanes : Anti-Ikaros antibody (ab168757) at 1 µg/ml

    Lane 1 : Ikaros-transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 53 kDa

References

ab168757 has not yet been referenced specifically in any publications.

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