Anti-IKB alpha antibody (ab7217)
Key features and details
- Rabbit polyclonal to IKB alpha
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IKB alpha antibody
See all IKB alpha primary antibodies -
Description
Rabbit polyclonal to IKB alpha -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein (Mouse).
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Positive control
- WB: mouse brain tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
Target
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Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. -
Involvement in disease
Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant -
Sequence similarities
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats. -
Post-translational
modificationsPhosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. - Information by UniProt
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Database links
- Entrez Gene: 18035 Mouse
- SwissProt: Q9Z1E3 Mouse
- Unigene: 170515 Mouse
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Alternative names
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (73)
ab7217 has been referenced in 73 publications.
- Liu W et al. Suppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression. J Cancer 13:1958-1971 (2022). PubMed: 35399722
- Peppicelli S et al. Extracellular Acidosis Differentially Regulates Estrogen Receptor β-Dependent EMT Reprogramming in Female and Male Melanoma Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36499700
- Sun X et al. Gut Microbiota and SCFAs Play Key Roles in QingFei Yin Recipe Anti-Streptococcal Pneumonia Effects. Front Cell Infect Microbiol 11:791466 (2021). PubMed: 34950611
- Sun X et al. Activation of Autophagy Through the NLRP3/mTOR Pathway: A Potential Mechanism for Alleviation of Pneumonia by QingFei Yin. Front Pharmacol 12:763160 (2021). PubMed: 35111047
- Galinski B et al. XPO1 inhibition with selinexor synergizes with proteasome inhibition in neuroblastoma by targeting nuclear export of IkB. Transl Oncol 14:101114 (2021). PubMed: 33975179