Western blot abreview for Anti-IKB alpha antibody [E130]

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hepatocytes)
Loading amount
50 µg
Specification
Hepatocytes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/5000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS T20

Secondary antibody

Name
Non-Abcam antibody was used: swine anti-rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: approximately 37 kDa

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Submitted Dec 06 2012

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