Western blot abreview for Anti-IKB alpha antibody [E130]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (smooth muscle cells)
Loading amount
25 µg
Specification
smooth muscle cells
Treatment
infection with interested gene for 48 hours
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk powder in TBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa

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Verified customer

Submitted Jan 16 2013

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