Western blot

Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+0.1% Tween 20
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: swine anti-rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 36 kDa
Exposure
5 minute(s) and 0 second(s)

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Submitted Jun 17 2013

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