Western blot abreview for Anti-IKB alpha antibody [E130]

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle (AT))
Specification
Skeletal muscle (AT)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.005%)
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Bands
Specific: 36 kDa
Exposure
30 second(s)

Additional data

Additional Notes
Representative western blot of IKB alpha protein from AT skeletal muscles of young WT mice.

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Submitted Mar 27 2014

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