Western blot abreview for Anti-IKB alpha antibody [E130]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human head and neck cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
10 nM PMA
Specification
human head and neck cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti-rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 37 kDa

Abcam user community

Verified customer

Submitted Aug 31 2015

Sign up