Western blot abreview for Anti-IKB alpha antibody [E130]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
28 min IRI
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Jackson Goat Anti-Rabbit IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 37 kDa

Additional data

Additional Notes
PVDF membrane

Dr. Wesley Konsavage

Verified customer

Submitted Sep 15 2015

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