Western blot abreview for Anti-IKB alpha antibody [E130]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Endothelial)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Endothelial
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Enzo lifescience
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
1 second(s)
Bands
Specific: 36 kDa

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Submitted Jul 28 2017

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