Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% gradient)
Loading amount
30 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL1
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 27 2019