Application
Western blot
Sample
Human Cell lysate - whole cell (Stroma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
250000 cells
Treatment
transfected with construct
Specification
Stroma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS +1% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Detection
Detection method
Fluorescent Detection
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Additional data
Additional Notes
the type of constructs are siRNA used for the knock down (KD)
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Submitted May 12 2022