Recombinant
RabMAb

Recombinant Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free (ab239920)

Overview

  • Product name

    Anti-IKB alpha (phospho S32) antibody [EPR3148] - BSA and Azide free
    See all IKB alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR3148] to IKB alpha (phospho S32) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IKB alpha (phospho S32). The exact sequence is proprietary.

  • General notes

    ab239920 is the carrier-free version of ab92700 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239920 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
    • Involvement in disease

      Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
    • Sequence similarities

      Belongs to the NF-kappa-B inhibitor family.
      Contains 5 ANK repeats.
    • Post-translational
      modifications

      Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
      Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
      Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
      Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
    • Information by UniProt
    • Database links

    • Alternative names

      • I kappa B alpha antibody
      • I-kappa-B-alpha antibody
      • IkappaBalpha antibody
      • IkB-alpha antibody
      • IKBA antibody
      • IKBA_HUMAN antibody
      • IKBalpha antibody
      • MAD 3 antibody
      • MAD3 antibody
      • Major histocompatibility complex enhancer-binding protein MAD3 antibody
      • NF kappa B inhibitor alpha antibody
      • NF-kappa-B inhibitor alpha antibody
      • NFKBI antibody
      • NFKBIA antibody
      • Nuclear factor of kappa light chain gene enhancer in B cells antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
      see all

    Images

    • ab92700 at 1/20 dilution immunoprecipitating IKB alpha (phospho S32) in HeLa (human cervix adenocarcinoma) treated with 20ng/mL TNF-alpha for 60 minutes, whole cell lysate, observed at 36 kDa (lanes 1 and 2).

      Lane 1 (input): HeLa treated with 20ng/mL TNF-alpha for 60 minutes, whole cell lysate, 10μg.

      Lane 2 (+): ab92700 + HeLa treated with 20ng/mL TNF-alpha for 60 minutes, whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92700 in HeLa treated with 20ng/mL TNF-alpha for 60 minutes, whole cell lysate.

      For western blotting, ab92700 at 1/200 dilution followed by VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92700).

    • Dot blot analysis of IKB alpha (phospho S32) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labeling IKB alpha (phospho S32) with ab92700 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/10000 was used as the secondary antibody.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92700).

    References

    ab239920 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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