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    ikb-alpha-phospho-y305-antibody-ab24784.pdf

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Anti-IKB alpha (phospho Y305) antibody (ab24784)

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Western blot - Anti-IKB alpha (phospho Y305) antibody (ab24784)

    Key features and details

    • Rabbit polyclonal to IKB alpha (phospho Y305)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Protein
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    Recombinant Human IKB alpha protein (ab113133)
    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-IKB alpha (phospho Y305) antibody
      See all IKB alpha primary antibodies
    • Description

      Rabbit polyclonal to IKB alpha (phospho Y305)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human IKB alpha (phospho Y305) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 305 of human IKB alpha. This peptide sequence has low homology to other IKB proteins.

    • Positive control

      • A431 and Jurkat cells treated with pervanadate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho IKB alpha (Tyr-305) peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • NFkB pathway
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • NFkB pathway
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human IKB alpha protein (ab113133)

    Applications

    Our Abpromise guarantee covers the use of ab24784 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500. Detects a band of approximately 38 kDa.

    Target

    • Function

      Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
    • Involvement in disease

      Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
    • Sequence similarities

      Belongs to the NF-kappa-B inhibitor family.
      Contains 5 ANK repeats.
    • Post-translational
      modifications

      Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
      Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
      Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
      Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
    • Target information above from: UniProt accession P25963 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4792 Human
      • Omim: 164008 Human
      • SwissProt: P25963 Human
      • Unigene: 81328 Human
      • Alternative names

        • I kappa B alpha antibody
        • I-kappa-B-alpha antibody
        • IkappaBalpha antibody
        • IkB-alpha antibody
        • IKBA antibody
        • IKBA_HUMAN antibody
        • IKBalpha antibody
        • MAD 3 antibody
        • MAD3 antibody
        • Major histocompatibility complex enhancer-binding protein MAD3 antibody
        • NF kappa B inhibitor alpha antibody
        • NF-kappa-B inhibitor alpha antibody
        • NFKBI antibody
        • NFKBIA antibody
        • Nuclear factor of kappa light chain gene enhancer in B cells antibody
        • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
        see all

      Images

      • Western blot - Anti-IKB alpha (phospho Y305) antibody (ab24784)
        Western blot - Anti-IKB alpha (phospho Y305) antibody (ab24784)
        Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab24784? Please let us know so that we can cite the reference in this datasheet.

      ab24784 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-IKB alpha (phospho Y305) antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Deer mouse Tissue lysate - whole (Liver)
      Gel Running Conditions
      Non-reduced Denaturing
      Loading amount
      20 µg
      Specification
      Liver
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 28 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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