Anti-IKB alpha (phospho Y42) antibody (ab24783)
Key features and details
- Rabbit polyclonal to IKB alpha (phospho Y42)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-IKB alpha (phospho Y42) antibody
See all IKB alpha primary antibodies -
Description
Rabbit polyclonal to IKB alpha (phospho Y42) -
Host species
Rabbit -
Specificity
There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IKB alpha (phospho Y42) conjugated to keyhole limpet haemocyanin.
-
General notes
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.Useful in detection of phosphorylated IKB alpha (Tyr-42).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier. -
Primary antibody notes
Useful in detection of phosphorylated IKB alpha (Tyr-42). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). |
Target
-
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. -
Involvement in disease
Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant -
Sequence similarities
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats. -
Post-translational
modificationsPhosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. - Information by UniProt
-
Database links
- Entrez Gene: 4792 Human
- Omim: 164008 Human
- SwissProt: P25963 Human
- Unigene: 81328 Human
-
Alternative names
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
see all
Images
-
Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.
Datasheets and documents
-
SDS download
-
Datasheet download
References (19)
ab24783 has been referenced in 19 publications.
- Shi CX et al. Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia-reperfusion rats. Mol Med Rep 21:123-130 (2020). PubMed: 31746402
- Luo Y et al. Taurine attenuates liver autophagy and injury of offspring in gestational diabetic mellitus rats. Life Sci 257:117889 (2020). PubMed: 32502541
- Lv H et al. Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429. Cell Cycle 19:207-217 (2020). PubMed: 31876239
- Meng Y et al. Knockdown of exosome-mediated lnc-PVT1 alleviates lipopolysaccharide-induced osteoarthritis progression by mediating the HMGB1/TLR4/NF-?B pathway via miR-93-5p. Mol Med Rep 22:5313-5325 (2020). PubMed: 33174011
- Li T et al. Silencing cyclophilin A improves insulin secretion, reduces cell apoptosis, and alleviates inflammation as well as oxidant stress in high glucose-induced pancreatic ß-cells via MAPK/NF-kb signaling pathway. Bioengineered 11:1047-1057 (2020). PubMed: 32970961