Recombinant
RabMAb

Recombinant Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685)

Overview

  • Product name

    Anti-IKB alpha (phospho Y42) antibody [EPR2353]
    See all IKB alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR2353] to IKB alpha (phospho Y42)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human IKB alpha aa 1-100 (phospho Y42).
    Database link: P25963

  • Positive control

    • Lysate from A431 cells pre-treated with pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
    • Involvement in disease

      Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
    • Sequence similarities

      Belongs to the NF-kappa-B inhibitor family.
      Contains 5 ANK repeats.
    • Post-translational
      modifications

      Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
      Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
      Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
      Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
    • Information by UniProt
    • Database links

    • Alternative names

      • I kappa B alpha antibody
      • I-kappa-B-alpha antibody
      • IkappaBalpha antibody
      • IkB-alpha antibody
      • IKBA antibody
      • IKBA_HUMAN antibody
      • IKBalpha antibody
      • MAD 3 antibody
      • MAD3 antibody
      • Major histocompatibility complex enhancer-binding protein MAD3 antibody
      • NF kappa B inhibitor alpha antibody
      • NF-kappa-B inhibitor alpha antibody
      • NFKBI antibody
      • NFKBIA antibody
      • Nuclear factor of kappa light chain gene enhancer in B cells antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
      see all

    Images

    • All lanes : Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685) at 1/500 dilution

      Lane 1 : lysate from untreated A431 cells
      Lane 2 : lysate from A431 cells
      pre-treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 36 kDa

    References

    ab92685 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your reply. Regarding ab92685, our product datasheet is showing that the antibody should be stored at -20. We recommend aliquoting to reduce the number of freeze thaw cycles, but if you were to thaw and aliquot the antibody in 10ul aliquots, we do not expect that this would dramatically affect the quality of the antibody. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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