For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    ikb-beta-antibody-epr5037-ab109509.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IKB beta antibody [EPR5037] (ab109509)

  • Datasheet
Reviews (1) Submit a question References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Immunoprecipitation - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Flow Cytometry (Intracellular) - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
  • Anti-IKB beta antibody [EPR5037] (ab109509)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5037] to IKB beta
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human IKB beta protein (ab85246)
Primary
Product image
Anti-VEGFC antibody (ab9546)

View more associated products

Overview

  • Product name

    Anti-IKB beta antibody [EPR5037]
    See all IKB beta primary antibodies
  • Description

    Rabbit monoclonal [EPR5037] to IKB beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HeLa, THP-1, and MCF7 cell lysates. IP: HeLa cells. IHC-P: Human kidney tissue. ICC/IF: Jurkat cells. Flow Cyt (intra): Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5037
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Anti-IKB beta antibody [EPR5037] - BSA and Azide free (ab247886)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • THP-1 nuclear extract lysate (ab14900)
  • Recombinant Protein

    • Recombinant Human IKB beta protein (ab85246)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250.

Flow Cyt (Intra)
1/20.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 37 kDa).
IP
1/10 - 1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Notes
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250.

Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 37 kDa).
IP
1/10 - 1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Target

  • Function

    Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • Tissue specificity

    Expressed in all tissues examined.
  • Sequence similarities

    Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications

    Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q15653 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4793 Human
    • Omim: 604495 Human
    • SwissProt: Q15653 Human
    • Unigene: 9731 Human
    • Alternative names

      • I kappa B beta antibody
      • I-kappa-B-beta antibody
      • IkappaBbeta antibody
      • IKB beta antibody
      • IkB-B antibody
      • IkB-beta antibody
      • IKBB antibody
      • IKBB_HUMAN antibody
      • IkBbeta antibody
      • NF kappa BIB antibody
      • NF-kappa-B inhibitor beta antibody
      • NF-kappa-BIB antibody
      • Nfkbib antibody
      • Thyroid receptor interacting protein 9 antibody
      • Thyroid receptor-interacting protein 9 antibody
      • TR interacting protein 9 antibody
      • TR-interacting protein 9 antibody
      • TRIP-9 antibody
      • TRIP9 antibody
      see all

    Images

    • Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
      Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
      Anti-IKB beta antibody [EPR5037] (ab109509) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 37 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
      Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: IKB beta knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - unpurified ab109509 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab109509 was shown to specifically react with IKB beta in wild type cells as signal was lost in IKB beta knockout cells. Wild-type and IKB beta knockout samples were subjected to SDS-PAGE. ab109509 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunoprecipitation - Anti-IKB beta antibody [EPR5037] (ab109509)
      Immunoprecipitation - Anti-IKB beta antibody [EPR5037] (ab109509)

      ab109509 (purified) at 1:20 dilution (1 µg) immunoprecipitating IKB beta in HeLa whole cell lysate.

      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab109509 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109509 in HeLa whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-IKB beta antibody [EPR5037] (ab109509)
      Flow Cytometry (Intracellular) - Anti-IKB beta antibody [EPR5037] (ab109509)

      Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IKB beta with purified ab109509 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-IKB beta antibody [EPR5037] (ab109509)
      Immunocytochemistry/ Immunofluorescence - Anti-IKB beta antibody [EPR5037] (ab109509)

      Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IKB beta with purified ab109509 at 1:50 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB beta antibody [EPR5037] (ab109509)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB beta antibody [EPR5037] (ab109509)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling IKB beta with purified ab109509 at 1:500 dilution (0.20 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB beta antibody [EPR5037] (ab109509)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB beta antibody [EPR5037] (ab109509)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue using unpurified ab109509 at a dilution of 1/100.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
      Western blot - Anti-IKB beta antibody [EPR5037] (ab109509)
      All lanes : Anti-IKB beta antibody [EPR5037] (ab109509) at 1/1000 dilution (Unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?

    • Anti-IKB beta antibody [EPR5037] (ab109509)
      Anti-IKB beta antibody [EPR5037] (ab109509)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (7)

    Publishing research using ab109509? Please let us know so that we can cite the reference in this datasheet.

    ab109509 has been referenced in 7 publications.

    • Shen L  et al. A naturally derived small molecule NDSM253 inhibits IKK1 to suppress inflammation response and promote bone healing after fracture. Am J Transl Res 13:24-37 (2021). PubMed: 33527006
    • Xiao N  et al. Egg yolk oils exert anti-inflammatory effect via regulating Nrf2/NF-?B pathway. J Ethnopharmacol 274:114070 (2021). PubMed: 33785356
    • Zhao L  et al. TNF-a promotes insulin resistance in obstructive sleep apnea-hypopnea syndrome. Exp Ther Med 21:568 (2021). PubMed: 33850540
    • Liu J  et al. Long noncoding RNA LINC01578 drives colon cancer metastasis through a positive feedback loop with the NF-?B/YY1 axis. Mol Oncol 14:3211-3233 (2020). PubMed: 33040438
    • Hsueh YS  et al. Nuclear KIT induces a NFKBIB-RELA-KIT autoregulatory loop in imatinib-resistant gastrointestinal stromal tumors. Oncogene 38:6550-6565 (2019). PubMed: 31363162
    • Wen H  et al. Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma. Nat Med 24:154-164 (2018). WB . PubMed: 29291352
    • Yi L  et al. Inflammation-mediated SOD-2 upregulation contributes to epithelial-mesenchymal transition and migration of tumor cells in aflatoxin G1-induced lung adenocarcinoma. Sci Rep 7:7953 (2017). PubMed: 28801561

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-IKB beta antibody [EPR5037]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
    Read More

    DR. Robert Kupp

    Verified customer

    Submitted Sep 17 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.